Key features and details | |
Cat. No. | MABL-1349 |
Name | Anti-Cytotoxin 1, 3, 7 mAbs |
Clone No. | AFD- TPL0027_01_F7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Naja nigricollis |
Basic Information | |
Specificity | TPL0027_01_F7 antibody recognizes cytotoxins 1, 3 and 7, which are some of the major toxins in the venom of the black-necked spitting cobra (Naja nigricollis). |
Alternative Name | CTX1,3,7; CX1; Cardiotoxin F8; Cardiotoxin-I; CTX-I; Toxin CM-6; Cytotoxin 3; CX3; Toxin CM-7; Cytotoxin 7; Toxin CM-4B |
UniProt | |
Immunogen | The antibody was raised using IONTAS phage displa library, a naïve human scFv library consisting of 4.0 · 10^10 unique antibody fragments. Through phage display selection, scFv-presenting phages were allowed to bind a toxin, phages with unspecific binding were washed away, and the rest were eluted through enzymatic digestion. These were then amplified and used for the following round. In total three rounds of selections were completed leaving the best binders behind. |
Application Notes | TPL0027_01_F7 antibody has high affinity to the cytotoxins 1, 3 and 7. It was demonstrated by normalized ELISA, which circumvents the limitation of a simple ELISA by removing the expression variable, normalizing the amount of scFv in each well. The antibody's high affinity was confirmed by its ability to bind the target proteins in a venom fraction even at 1:32,000 dilution (3.125 · 10^-2) µg/mL (Jürgensen et al., 2019 [poster]). It is recommended for the research on the antitoxin against Naja nigricollis snakebites. |
Antibody First Published | Jürgensen et al. Harnessing monoclonal antibodies for development of a specific treatment against Naja nigricollis envenoming. 10.13140/RG.2.2.24771.14884. |
Note on publication | This poster describes the generation and characterization of the TPL0027_01_F7 antibody among other antibodies directed against the venom toxins of Naja nigricollis. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
