Key features and details | |
Cat. No. | MABL-1328 |
Name | Anti-Cytokeratin 14 mAbs |
Clone No. | AFD- RCK107 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, FC, IHC |
Species Reactivity | canine, Rat, Swine, Human |
Basic Information | |
Specificity | The antibody reacts exclusively with Cytokeratin 14 which is present in basal cell compartments of stratified and combined epithelia. |
Alternative Name | K14; CK-14; Keratin K14; Keratin type I cytoskeletal 14; Cytokeratin-14; Keratin-14 |
UniProt | P02533 |
Immunogen | The original antibody was generated by immunizing a mouse with a cytoskeletal preparation of TR146 epithelial cells. |
Application Notes | The antibody detected keratin-14 from cytoskeletal extracts were prepared from A431 and HaCaT cell cultures by western blot analysis. This antibody was used for immunohistochemistry studies of keratins on TR146, HaCaT and A431 cells. The antibody stained mostly basal (myoepithelial) cells (Wetzels et al., 1991; PMID： 1705754 and Smedts et al., 1992; PMID： 1379783). Further, the antibody stained mitochondria- rich cells (Spies et al., 1997; PMID： 9440221). |
Antibody First Published | Wetzels et al. Basal cell-specific and hyperproliferation-related keratins in human breast cancer Am J Pathol. 1991 Mar;138(3)：751-63. PMID：1705754 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
