Key features and details | |
Cat. No. | MABL-1291 |
Name | Anti-CTLA4 mAbs |
Clone No. | AFD- 1B8 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, WB, ELISA, FC, IF |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody is specific for CTLA-4. CTLA-4 is an inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. |
Alternative Name | Cytotoxic T-lymphocyte protein 4; Ctla4; Cytotoxic T-lymphocyte- associated antigen 4; CTLA-4; CD152; UC10-4FIO |
UniProt | P09793 |
Immunogen | The antibody was generated by immunizing Armenian hamsters with the extracellular portion of murine CTLA-4 fused to a murine IgG2. |
Application Notes | ELISA was preformed on murine CTLA-4lg to test the binding specificity of the anitbody (Walunas et al, 1994; pmid:7882171). For flow cytometry on A20, M12.41 (B cells), L cell (fibroblast), and P815 (mastocytoma) cells this antibody was used (Perkins et al, 1996; pmid:8666782). This antibody was used for blocking in mice. It prevented T-cell proliferation (Leach et al, 1996; pmid: 8596936). Western blot was preformed on LN cells harvested from SJL mice using this antibody (Aruna et al, 2005; pmid: 16014414). This antibody was used for flow cytometry on LN cells harvested from SJL mice (Aruna et al, 2005; pmid: 16014414). Mouse CD4+ CD25+ T cells were used for a flow cytomic experiment using this antibody (Valsazina et al, 2006; pmid: 16618776). |
Antibody First Published | Walunas et al. CTLA-4 can function as a negative regulator of T cell activation Immunity. 1994 Aug;1(5): 405-13. PMID: 7882171 |
Note on publication | Describes the generation of the antibody by immunizing Armenian hamsters with the extracellular portion of murine CTLA-4 fused to a murine IgG2. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
