Key features and details | |
Cat. No. | MABL-1245 |
Name | Anti-Complement C3 mAbs |
Clone No. | AFD- MA11 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA; |
Species Reactivity | Guinea Pig |
Basic Information | |
Specificity | MA11 is highly specific for guinea pig C3, with no binding observed between MA11 and guinea pig C3a, rat C3 and human C3. |
Alternative Name | C3 |
UniProt | P12387 |
Immunogen | A female New Zealand White rabbit was immunised with guinea pig C3 by three subsequent subcutaneous injections. The RNA was isolated from the spleen, a phage display library constructed and then used to produce soluble scFv fragments. |
Application Notes | ELISA was used to confirm the specificity of MA11 for guinea pig C3, with MA11 displaying high reactivity for gp C3. A higher signal intensity is observed with MA11 than with MF9. Reactivity with guinea pig C3a, rat C3, and human C3 was not observed (Hawlisch et al, 2000). |
Antibody First Published | Hawlisch et al, 2000. Guinea pig C3 specific rabbit single chain Fv antibodies from bone marrow, spleen and blood derived phage libraries J Immunol Methods. 2000 Mar 6;236(1-2):117- 31 PMID:10699585 |
Note on publication | Describes the original generation of MA11 and its characterisation through ELISA. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
