Anti-CD98 heavy chain mAbs

Cat. No.

MABL-1169

Name

Anti-CD98 heavy chain mAbs

Clone No.

AFD- HBJ127

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Activate, Block, ELISA, IF

Species Reactivity

Human

Basic Information

Specificity

HBJ127 binds to the CD98 heavy chain at an   epitope consisting of residues AFS (aa 442-444) (Itoh et al, 2007). The   antibody has not been shown to cross-react with other species. CD98 is   expressed at high levels on monocytes and at very low level on peripheral   blood T and B lymphocytes, splenocytes, NK cells and granulocytes. CD98 is a   disulfide-linked and glycosylated type II integral membrane protein. This   protein has roles in normal and neoplastic cell growth and is required for   the function of light chain amino acid transporters. It is also involved in   sodium-independent transport of large neitral amino acids and in guiding and   targeting LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6   or SLC7A7, CD98 acts as an arginine/glutamine exchanger.

Alternative Name

4F2hc; HBJ 127; HBJ-127; CD98; GP125; 4F2   cell-surface antigen heavy chain; 4F2 heavy chain antigen; Lymphocyte   activation antigen 4F2 large subunit; Solute carrier family 3 member 2

UniProt

P08195

Immunogen

HBJ127 was obtained from hybridomas generated by the fusion of mouse myeloma cells and spleen cells from mice which had   been immunized with T24 hyman bladder cancer cells.

Application Notes

HBJ127 can inhibit lymphocyte activation and   proliferation in vitro (Yagita and Hashimoto, 1986), as well as inhibit the   growth of human bladder tumors (Yagita, 1986). The antibody also mediates the   inhibition of DNA synthesis of Con A-stimulated lymphocytes at concentrations   greater than 13 μg/ml, the inhibition of mAB 6-1-13-induced c-src expression   and the inhibition of mAb 6-1-13-induced polykaryocyte formation. In   addition, HBJ127 can enhance expression of the mannose receptor on the   surface of monocytes derived from healthy humans. HBJ127 shows positive   staining of murine L929 cells transiently expressing human CD98 heavy chain   in IF studies. The antibody can also be used to detect CD98 heavy chain by   ELISA.

Antibody First Published

Yagita et al. Monoclonal antibodies that   inhibit activation and proliferation of lymphocytes. I. Expression of the   antigen on monocytes and activated lymphocytes. J Immunol. 1986 Mar   15;136(6):2055-61. PMID:3950410

Note on publication

Describes the in vtro effect of HBJ127 and   other mAbs on lymphocyte activation and proliferation, and gave evidence to  suggdst that the HBJ127 antigen was important in the activation of   lymphocytes and its subsequent progression through the cell cycle.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.