Key features and details | |
Cat. No. | MABL-1143 |
Name | Anti-CD86 mAbs |
Clone No. | AFD- PO3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, functional assays, WB, FC, IHC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody is specific for murine CD86, an ~80 kDa surface receptor of the B7 family of costimulatory molecules. In addition to CD80, CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). This interaction plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. |
Alternative Name | PO.3; PO3.1; B7-2; Activation B7-2 antigen; B-lymphocyte activation antigen B7-2; B-lymphocyte antigen B7-2; B7.2; CD28 antigen ligand 2; CD28LG2; CD86; early T cell costimulatory molecule-1; early T-cell costimulatory molecule 1; ETC-1; LAB72; MGC34413; T-lymphocyte activation antigen CD86 |
UniProt | P42082 |
Immunogen | This antibody was raised by immunising Sprague Dawley rats with a mouse B cell line, BCL1 and fusing immune splenocytes with P3U1 myeloma cells. |
Application Notes | This antibody has been used in multiple FACS experiments for various immunological applications, such as to demonstrate how the C-type lectin receptor DCIR modulates immunity to tuberculosis (Troegeler et al., 2017), to elucidate the mechanism of Th17 cell differentiation by cholera toxin (Kang et al., 2016), and to evaluate the role of the spleen tyrosine kinase in graft-versus-host disease (Flynn et al., 2015). This antibody has also been used in immunofluorescence to investigate the potential of peritoneal cavity B cells to alleviate murine colitis (Maseda et al., 2013) and in Western blot to study the effect of interleukin 4-induced gene-1 (IL4I1) overexpression on the expression of M2 markers and M1- associated cytokines (Yue et al., 2015). |
Antibody First Published | Atsuo Nakajima et al. Preferential dependence of autoantibody production in murine lupus on CD86 costimulatory molecul Eur J Immunol. 1995 Nov;25(11)：3060-9. PMID：7489744 |
Note on publication | Describe the use of this antibody, together with the anti-CD80 antibody, to confirm the preferential dependence of murine Ab-mediated autoimmunity on the CD86 costimulatory molecule. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
