Cat. No.
MABL-351
Application
WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
5F6
From
Recombinant Antibody
Specificity
This antibody is specific for acrolein-modified proteins. Protein modification by acrolein is largely attributed to the formation of acrolein-lysine and acrolein-histidine adducts that possess a carbonyl function. While the antibody has been raised against acrolein-modified KLH, it has been shown to recognize both acrolein-modified BSA and oxidized LDL.
Alternative Names
Acrolein modified protein; ACR-P; ACRL
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c mice with an acrolein-treated keyhole limpet hemocyanin (KLH).
Application Notes
A direct ELISA examined the immunoreactivity of this antibody to aldehyde-treated proteins. It was scarcely inhibited by Na -acetyl-Nim-propanal-histidine and significantly inhibited by Na - acetyl-FDP-lysine, indicating that FDP-lysine (Nε -(3-formyl-3,4-dehydropiperidino)lysine) is an epitope. This antibody was also used for immunohistochemistry (IHC) detection of protein-bound acrolein in fatty streak lesions of arterial tissue (Uchida et al., 1998; PMID: 9560197). This antibody was used in flow cytometry (FC) to detect protein-bound acrolein in activated human T cells (Fonseca et al., 2001; PMID: 11342443). A novel pyridinium-type lysine adduct, Nϵ -(3-methyl-pyridinium)lysine (MP-lysine), was investigated and found to be a major epitope of this antibody (Furuhata et al., 2003; PMID: 14504272). This antibody was used for IHC to detect oxidative stress in the testis of rats (Iuchi et al., 2004; PMID: 14673659). This antibody was used for WB to detect acrolein modification of apoE3-NT (Tamamizu-Kato et al., 2007; PMID: 17580963).
Antibody First Published
Uchida et al. Protein-bound acrolein: potential markers for oxidative stress. Proc Natl Acad Sci USA. 1998 Apr 28;95(9):4882-7. doi: 10.1073/pnas.95.9.4882 PMID:9560197
Note on publication
The original publication describes the development of the antibody, which recognizes protein-bound acrolein, a potential marker of oxidative stress and long-term protein damage inaging, atherosclerosis, and diabetes.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
