Cat. No.
MABL-293
Application
RIA, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
7D7 (7D7.2.3)
From
Recombinant Antibody
Specificity
This antibody binds 17α-hydroxyprogesterone (17-OHP).
Alternative Names
17-OH; 17-OHP; 17α-hydroxyprogesterone
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c mice with 17-OHP-bovine serum albumin conjugate as an immunogen.
Application Notes
This antibody binds 17-OHP, a chemical intermediate involved in the biosynthesis of many endogenous steroids like androgens, estrogens, glucocorticoids, and mineralocorticoids, as well as neurosteroids. Congenital adrenal hyperplasia (CAH) is an autosomal recessive disorder caused by the deficiency of one of the five enzymes involved in the biosynthesis of corticosteroids. The most common form of the disease is the deficiency of 21-hydroxylase that catalyses the production of cortisol. Due to lack of this enzyme there is an accumulation of high levels of 17α-hydroxyprogesterone (17-OHP). This antibody is therefore recommended for detection of high levels of 17-OHP caused by CAH. Fluorescence enzyme immunoassay and micro-enzyme immunoassay for 17-OHP was used to test the cross reactivity of this antibody with other steroids (PMID: 3312820).
Antibody First Published
Sawada et al. Production and characterization of monoclonal antibodies to 17 alpha-hydroxyprogesterone. J Steroid Biochem. (1987); 28(4):405-10. PMID:3312820
Note on publication
Describes the generation of this antibody and its cross reactivity with other steroids. A micro enzyme immunoassay was shown to be applicable for the screening of congenital adrenal hyperplasia.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
