Cat. No.
MAK-0129
Product Name
Horse IgG(T)
Storage
2-8°C
Species
Horse
Instructions
Four major IgG subisotypes are found in horse serum: IgGa, IgGb, IgGc and IgG(T). IgG(T) is reportedly the second most abundant isotype.
Introduction
Four major IgG subisotypes are found in horse serum: IgGa, IgGb, IgGc and IgG(T). IgG(T) is reportedly the second most abundant isotype. This ELISA kit is designed for measurement of IgG(T) in horse serum or plasma. The assay uses goat anti-horse IgG(T) for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated goat anti-horse IgG(T) antibodies for detection. Both capture and detection antibodies react specifically with IgG(T). Cross-reactivity with immunoglobulins from other species has not been investigated.
Principle Of The Assay
Test samples are diluted and incubated in the microtiter wells for 45 minutes alongside horse IgG(T) standards. The microtiter wells are subsequently washed and HRP conjugate is added and incubated for 45 minutes. IgG(T) molecules are thus sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-labeled antibodies and TMB Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow, and optical density is measured spectrophotometrically at 450 nm. The concentration of IgG(T) is proportional to the optical density of the test sample and is derived from a standard curve.
