Cat. No.
MAK-0095
Product Name
Monkey IgE
Storage
4°C
Species
Monkey
Instructions
The monkey IgE ELISA kit is designed for measurement of IgE in rhesus and cynomolgus monkey serum.
Introduction
The monkey IgE ELISA kit is designed for measurement of IgE in rhesus and cynomolgus monkey serum. One is used for solid phase immobilization (microtiter wells). The other, conjugated to HRP, is used for detection. IgE is the least abundant immunoglobulin in serum, typically present at levels of approximately 100 ng/ml . It is involved in allergic reactions, binding to Fc receptors on basophils and mast cells. Subsequent binding of antigen to IgE triggers release of histamine and other vasoactive amines. IgE levels are elevated in asthma, eczema, rhinitis, and parasitic infections.
Principle Of The Assay
Diluted serum samples and standards are incubated in the microtiter wells for one hour with HRP conjugate. If IgE molecules are present, they are sandwiched between the immobilization and detection antibodies. The wells are then washed. TMB reagent is added and incubated for 20 minutes. This results in the development of a blue color if IgE is present. Color development is stopped by the addition of Stop Solution, changing the color to yellow. Absorbance is measured at 450 nm. The concentrations of IgE in samples are proportional to absorbance and are derived from a standard curve.
