Cat. No.
MAK-0153
Product Name
Goat CRP
Storage
2-8°C
Species
Goat
Introduction
CRP is an acute phase protein that is elevated in serum from most mammals because of infection and disease and can be used as a biomarker to evaluate health status.
Principle Of The Assay
The assay uses affinity purified goat CRP antibodies for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated goat CRP antibodies for detection. Standards and diluted samples are incubated in the microtiter wells for 45 minutes. The wells are subsequently washed. HRP conjugate is added and incubated for 45 minutes. This results in CRP molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-conjugate and
TMB is added and incubated for 20 minutes. If CRP is present a blue color develops. Color development is stopped by the addition of Stop solution, changing the color to yellow, and absorbance is measured at 450 nm. The concentration of CRP is proportional to absorbance and is derived from a standard curve.
