Cat. No.
MABL-3608
Application
BLI, confocal microscopy, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
iMab
From
Recombinant Antibody
Specificity
This antibody is specific for the DNA i-motif, a four-stranded DNA structure that forms in cytosine-rich regions and is potentially involved in regulating DNA function.
Alternative Names
intercalated motif; i-motif; intercalated DNA motif; I motif; DNA i-motif; human telomere i-motif ; hTelo i-motif; imotif; imotiv; imotive; i-motiv; i-motive
UniProt
Immunogen
The original antibody was selected from the Garvan-2 human scFv library via phage display.
Application Notes
The original human scFv version of this antibody was validated for its specificity towards various i-motif types through ELISA and bio-layer interferometry (BLI), with a dissociation constant (KD) of 59 nM. It selectively binds to i-motifs, including those in 1G22, 1A83, c-MYC, RET, and VEGF sequences, located in regions such as telomeres, centromeres, and proto-oncogene promoters.Importantly, this antibody does not interact with structures resembling i-motif DNA, such as DNA G4 quadruplex forms, double-stranded DNA (dsDNA), hairpin DNA, miRNA, or mutant human telomeres lacking i-motifs (Zeraati et al., 2018; PMID: 29686376). Notably, it does not bind to various DNA G4 quadruplex forms, including promoter G4s c-MYC44, BCL245, VEGF, telomeric 2JSM47, 2GKU, or aptamer G4 TBA. Additionally, the antibody has proven effective for immunofluorescence detection of i-motifs in multiple cell lines (MCF7, U2OS, and HeLa), confirming the presence of i-motif structures in human nuclei and their dependence on pH and cell-cycle stage (Zeraati et al., 2018; PMID: 29686376).
Antibody First Published
Zeraati et al. I-motif DNA structures are formed in the nuclei of human cells. Nat Chem. 2018 Jun;10(6):631-637. doi: 10.1038/s41557-018-0046-3. Epub 2018 Apr 23.PMID:29686376
Note on publication
This article describes the generation and characterisation of the iMab antibody fragment and its use in the detection of i-motifs in the nuclei of human cells.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
