Cat. No.
MABL-3603
Application
Dot, IP, IF
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
AB3/63.3
From
Recombinant Antibody
Specificity
The anti-5-Hydroxymethylcytosine antibody, clone AB3/63.3, detects 5-hydroxymethyl cytosine (hmc, 5hmc), but not 5-methylcytosine or unmethylated cytosine.
Alternative Names
5hmc; MABE176; 5hmeC; 5' hydroxymethyl; cytosine; 5'OH methyl cytosine; 5'OHmeC; 5hmc; 5-hmC
UniProt
Immunogen
5-Hydroxymethylcytosine conjugated to BSA.
Application Notes
This antibody can be used to detect 5hmC by immunofluorescence and dot blot. Zhang et al. (2014, PMID: 24427299) used the original rat IgG2a format (under the name MABE176) in an immunoprecipitation experiment.
Antibody First Published
Ficz et al. Dynamic regulation of 5-hydroxymethylcytosine in mouse ES cells and during differentiation. Nature. 2011 May 19;473(7347):398-402 PMID:21460836
Note on publication
Describes the generation and use in immunofluorescence of the original antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
